Expression of Bovine Cytokines in Escherichia coli.

Expression of bovine mitochondrial tRNASer GCU derivatives in Escherichia coli.

By replacing a stretch of five A-U base pairs in the acceptor stem with G-C pairs, mitochondrial tRNA-SerGCU lacking a D arm could be expressed in Escherichia coli cells in considerable amounts. The expressed tRNA with no modified nucleoside was serylated in vitro with the mitochondrial enzyme. The tRNASerGCU derivatives carrying identity elements for alanine tRNA and the related anticodons wer...

Advances and challenges in recombinant protein expression in Escherichia coli

expression of bovine neutrophil b-defensin bnbd2 gene in escherichia coli

molecular cloning and expression of bovine viral diarrhea virus nonstructural protein 3 in escherichia coli

background bovine viral diarrhea (bvd) is an economically important disease of cattle with a worldwide distribution. diagnosis of bvd relies on laboratory-based detections of its viral causing agent or virus specific antibodies. the most common laboratory method used for this purpose is the elisa. bovine viral diarrhea virus (bvdv) nonstructural protein 3 (ns3) is one of the most highly conserv...

Escherichia coli mastitis in the Dairy Bovine

Mastitis is a continuous concern for dairy producers in the US because of its economic consequences. Coliform mastitis accounts for 20-80% of acute clinical mastitis cases and includes gram-negative pathogens Escherichia coli, Klebsiella and Enterobacter species. Rapid identification of the causative organism is essential to implement a prudent treatment plan. Escherichia coli can be rapidly id...